hind iii (TaKaRa)
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hind iii
Hind Iii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hind+iii/Hind+III/us12630855-245-16-18
Average 99 stars, based on 9130 article reviews
Hind Iii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hind+iii/Hind+III/us12630855-245-16-18
Average 99 stars, based on 9130 article reviews
hind iii - by Bioz Stars,
2026-09
99/100 stars
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Expressing:Article Title: Effects of TRIB3 gene overexpression and interference on lipid metabolism in mammary epithelial cells of yaks Article Snippet: The empty plasmid was extracted from the bacterial solution of pcDNA3.1 (+) plasmid (V79020, Invitrogen) stored in the laboratory using the High purity plasmid medium kit (EM101-01, full Gold) after amplification. .. The eukaryotic expression vector pcDNA3.1 (+) and purified PCR products were then digested with Article Title: PRSS23 Promotes Ovarian Follicular Atresia in Wuding Chickens by Coordinately Suppressing Steroidogenesis and PI3K/AKT/mTOR Survival Signaling Article Snippet: For overexpression studies, the CDS of PRSS23 was amplified from Wuding chicken ovarian cDNA using gene-specific primers ( ) and subsequently cloned into the pMD-18T vector (TaKaRa). .. The insert was then excised by double digestion with Kpn I and Article Title: PRSS23 Promotes Ovarian Follicular Atresia in Wuding Chickens by Coordinately Suppressing Steroidogenesis and PI3K/AKT/mTOR Survival Signaling Article Snippet: For overexpression studies, the CDS of PRSS23 was amplified from Wuding chicken ovarian cDNA using gene-specific primers (Table S1) and subsequently cloned into the pMD-18T vector (TaKaRa). .. The insert was then excised by double digestion with Kpn I and Plasmid Preparation:Article Title: Effects of TRIB3 gene overexpression and interference on lipid metabolism in mammary epithelial cells of yaks Article Snippet: The empty plasmid was extracted from the bacterial solution of pcDNA3.1 (+) plasmid (V79020, Invitrogen) stored in the laboratory using the High purity plasmid medium kit (EM101-01, full Gold) after amplification. .. The eukaryotic expression vector pcDNA3.1 (+) and purified PCR products were then digested with Article Title: Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens Article Snippet: .. Plasmid pNZ8148 was double-digested with Nco I and Article Title: PfVPS4, an ESCRT AAA-ATPase, is essential for asexual proliferation and gametocyte sexual conversion in Plasmodium falciparum. Article Snippet: .. PCR products and the pET28a (+) vector were digested with BamHI and Article Title: Tandem amplification of the umpA allele contributes to ceftazidime-avibactam heteroresistance in clinical carbapenem-resistant Klebsiella pneumoniae isolates Article Snippet: .. The vector pBAD33 was digested with Article Title: PRSS23 Promotes Ovarian Follicular Atresia in Wuding Chickens by Coordinately Suppressing Steroidogenesis and PI3K/AKT/mTOR Survival Signaling Article Snippet: For overexpression studies, the CDS of PRSS23 was amplified from Wuding chicken ovarian cDNA using gene-specific primers ( ) and subsequently cloned into the pMD-18T vector (TaKaRa). .. The insert was then excised by double digestion with Kpn I and Article Title: PRSS23 Promotes Ovarian Follicular Atresia in Wuding Chickens by Coordinately Suppressing Steroidogenesis and PI3K/AKT/mTOR Survival Signaling Article Snippet: For overexpression studies, the CDS of PRSS23 was amplified from Wuding chicken ovarian cDNA using gene-specific primers (Table S1) and subsequently cloned into the pMD-18T vector (TaKaRa). .. The insert was then excised by double digestion with Kpn I and Purification:Article Title: Effects of TRIB3 gene overexpression and interference on lipid metabolism in mammary epithelial cells of yaks Article Snippet: The empty plasmid was extracted from the bacterial solution of pcDNA3.1 (+) plasmid (V79020, Invitrogen) stored in the laboratory using the High purity plasmid medium kit (EM101-01, full Gold) after amplification. .. The eukaryotic expression vector pcDNA3.1 (+) and purified PCR products were then digested with Polymerase Chain Reaction:Article Title: Effects of TRIB3 gene overexpression and interference on lipid metabolism in mammary epithelial cells of yaks Article Snippet: The empty plasmid was extracted from the bacterial solution of pcDNA3.1 (+) plasmid (V79020, Invitrogen) stored in the laboratory using the High purity plasmid medium kit (EM101-01, full Gold) after amplification. .. The eukaryotic expression vector pcDNA3.1 (+) and purified PCR products were then digested with Article Title: PfVPS4, an ESCRT AAA-ATPase, is essential for asexual proliferation and gametocyte sexual conversion in Plasmodium falciparum. Article Snippet: .. PCR products and the pET28a (+) vector were digested with BamHI and Article Title: Tandem amplification of the umpA allele contributes to ceftazidime-avibactam heteroresistance in clinical carbapenem-resistant Klebsiella pneumoniae isolates Article Snippet: .. The vector pBAD33 was digested with Gel Extraction:Article Title: Probiotic and antibacterial properties of recombinant Lactococcus lactis expressing the fusion antimicrobial peptides BMAP18-BSN37 in mice and chickens Article Snippet: .. Plasmid pNZ8148 was double-digested with Nco I and Over Expression:Article Title: Tandem amplification of the umpA allele contributes to ceftazidime-avibactam heteroresistance in clinical carbapenem-resistant Klebsiella pneumoniae isolates Article Snippet: .. The vector pBAD33 was digested with DNA Ligation:Article Title: Polypeptide having esterification activity for L-menthol and/or hydrolyzing activity for L-menthol ester Article Snippet: A linker sequence (Nco I, Hind III) was added by PCR amplification (PrimeSTAR GXL DNA Polymerase (TaKaRa)), primer (forward primer: 5′-TTTTCCATGGCTCGTTCTATGCGTTCTCG-3′: SEQ ID NO: 6, reverse primer: 5′-AAAAAAGCTTAAACACCCGCCAGTTTCAGACGG-3′: SEQ ID NO: 7)) using the total synthesized structural gene (LipA; SEQ ID NO: 4) as a template, and then purification (NucleoSpin Gel and PCR Clean-up (MACHEREY-NAGEL)) was performed to obtain a gene fragment (BCL-LipA). .. The gene fragment (BCL-LipA) and pETDuet-1 (Novagen) were treated with a restriction enzyme (Nco I (TaKaRa), Transformation Assay:Article Title: Polypeptide having esterification activity for L-menthol and/or hydrolyzing activity for L-menthol ester Article Snippet: A linker sequence (Nco I, Hind III) was added by PCR amplification (PrimeSTAR GXL DNA Polymerase (TaKaRa)), primer (forward primer: 5′-TTTTCCATGGCTCGTTCTATGCGTTCTCG-3′: SEQ ID NO: 6, reverse primer: 5′-AAAAAAGCTTAAACACCCGCCAGTTTCAGACGG-3′: SEQ ID NO: 7)) using the total synthesized structural gene (LipA; SEQ ID NO: 4) as a template, and then purification (NucleoSpin Gel and PCR Clean-up (MACHEREY-NAGEL)) was performed to obtain a gene fragment (BCL-LipA). .. The gene fragment (BCL-LipA) and pETDuet-1 (Novagen) were treated with a restriction enzyme (Nco I (TaKaRa), |